Podcast
Absolute Gene-ius
Hosted by Jordan Ruggieri, Lisa Crawford · EN
Inspiring stories and advice from dPCR gene-iuses.
28episodes
Episodes
Newest firstAll episodes
How PCR, CRISPR, and animal models are advancing oncology research
2w ago00:16:20Tap to summarizeCancer research is moving fast, and molecular tools are helping scientists see more, measure more, and ask better questions. In this Science Snapshot episode of Absolute Gene-ius, Jordan Ruggieri and Lisa Crawford revisit memorable oncology-related clips from past conversations. Dr. Raquel Munoz explains how CAR-T cells are engineered to recognize and kill cancer cells, and how PCR-based methods can help confirm successful modification and track CAR-T expansion. Dr. Dustin Rubinstein shares how CRISPR and genome editing are being used to create pig models, including “onco-pig,” that may better reflect aspects of human cancer biology than traditional models. The episode also connects cell-free DNA research from Dr. Lee Ann Baxter-Lowe to oncology applications, before turning to Dr. Kathie Sollweck’s discussion of circulating tumor DNA and the sensitivity needed to detect low mutational burden in liquid biopsy research. Finally, Valeria Rangel offers practical perspective on multiplex PCR assay design. And because it is Absolute Gene-ius, the science comes with some laughs, a few pig jokes, and a reminder that even complex oncology methods can be surprisingly fun to talk about. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.
Transcribe →Genotoxicity, vectors, and the power of integration site analysis
Jun 1700:35:15Tap to summarizeIn this episode, Michael Stump and Matteo Franco from ProtaGene explore the growing importance of integration site analysis (ISA) in cell and gene therapy. As viral vectors like lentivirus and AAV become more widely used, understanding where and how they integrate into the genome is essential for assessing genotoxicity and long-term safety. The discussion dives into how sequencing reveals integration locations, clonal expansion risks, and potential activation of oncogenes. They also highlight how tools like digital PCR complement sequencing as orthogonal methods that help enable precise quantification, validation, and new applications like vector integrity and copy number analysis. Beyond the science, the conversation explores the CRO role in supporting both early-phase discovery and late-stage regulatory needs, balancing innovation with regulatory expectations, and adapting to emerging modalities like LNPs. The career corner brings a mix of honesty and humor, from chaotic CRO workdays to broken PCR capillaries and runaway lab mice, paired with thoughtful advice on navigating industry paths, building GMP expertise, and finding the right fit between academia and industry. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.
Transcribe →From brewing to bioprocessing – Steve Lewis on molecular biology trends
Jun 800:22:45Tap to summarizePCR may be decades old, but it is still one of the most versatile tools in modern molecular biology. In this crossover episode, Absolute Gene-ius welcomes Steve Lewis, host of Speaking of Mol Bio and Director of Product Management at Thermo Fisher Scientific. Steve discusses why PCR remains so broadly relevant across research and applied science, from human identification and food testing to gene synthesis and molecular characterization. He also explores emerging trends shaping molecular biology workflows, including multiplexing, assay design, customization, miniaturization, and the ongoing need for high-quality PCR plastics. The conversation also digs into sustainability in the lab, including why single-use plastics remain essential for reducing contamination risk and how more sustainable material sourcing can help reduce carbon impact without compromising performance. In Career Corner, Steve shares a winding path that started in communications, moved through startups and home brewing, and eventually led to bioprocessing, biosecurity, synthetic biology, and product management. His advice? Cultivate curiosity, surround yourself with good people, and try not to drop your E. coli flask. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.
Transcribe →Epigenetics unlocked – how behavior shapes biology
May 2000:41:41Tap to summarizeDr. Robert Philibert joins the show to unpack the fascinating world of epigenetics, where environmental factors like smoking, alcohol, and lifestyle reshape how genes are expressed. He explains how methylation, histone modifications, and chromatin structure act as regulatory layers on top of DNA, influencing disease risk far more than previously thought. The conversation dives into how technologies like bisulfite conversion and digital PCR enable precise measurement of these changes, transforming epigenetic signals into actionable data. Dr. Philibert also highlights real-world applications, from detecting behavioral risk factors to enabling early disease biomarkers, emphasizing how epigenetics can’t be “gamed” like traditional reporting methods, making it a powerful tool for precision health interventions. From spilled drink mishaps to science fiction inspiration, the career corner brings humor and humanity. Dr. Philibert reflects on mentorship, passion, and the joy of seeing students turn ideas into impactful innovations, proving that science is as much about people as it is about discovery. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.
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Multiplexing in action – from simplicity to scientific complexity
May 600:19:05Tap to summarizeMultiplexing is reshaping how scientists think about PCR experiments, turning single-target workflows into powerful, multi-dimensional analyses. In this Science Snapshot, we revisit standout moments from past episodes to explore how multiplexing is applied across research areas, from measuring interferon-related genes in lupus to analyzing complex microbial communities and detecting low-level donor-derived DNA. Guests highlight both the opportunities and challenges, including assay design, probe optimization, and troubleshooting strategies. The episode also showcases how multiplexing enables entirely new applications, such as comparing chromosome ratios, assessing molecular integrity, and distinguishing functional mRNA from truncated byproducts. Whether using qPCR or digital PCR, multiplexing allows researchers to extract more information per sample, improve precision through multiple data points, and better reflect biological complexity. As platforms and chemistries continue to evolve, multiplexing is becoming more accessible, helping scientists move faster, conserve material, and focus more on interpreting results rather than optimizing reactions. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.
Transcribe →Finding the cheat codes to cell identity
Apr 1500:33:18Tap to summarizeIn this episode, Fiona Connolly, former Platform Innovation and Automation Scientist at bit.bio, explains how deterministic programming replaces the traditional “pinball machine” model of stem cell differentiation with precise genetic instructions. By identifying the exact transcription factor combinations, the “cheat codes” that define specific cell fates, Fiona’s team engineers induced pluripotent stem cells (iPSCs) to reliably become neurons, oligodendrocyte-like cells, and other specialized types. She discusses how digital PCR enables accurate transgene copy number validation and multiplexed screening of hundreds of clones, while RNA-seq and qPCR confirm expression fingerprints. The result? Consistent, scalable, high-quality human cell models that accelerate disease research, particularly in areas like neurodegeneration and multiple sclerosis while reducing reliance on animal models. In Career Corner, Fiona shares her journey from curious “why” kid to molecular biologist obsessed with CRISPR, robotics, and automation. Her advice: don’t over-optimize your career path. Stay pluripotent, explore interdisciplinary skills, embrace new tech, and let curiosity guide the way. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.
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Science happens, and so do mistakes
Apr 100:14:17Tap to summarizeScience doesn’t just advance through breakthroughs, it advances through mistakes. This Science Snapshot episode embraces a universal truth of research: if you haven’t messed something up in the lab, you probably haven’t been there very long. From chemical reactions gone wrong to experiments derailed by one small oversight, these stories are as relatable as they are unforgettable. In this curated “best of” revisit, Absolute Gene-ius brings together candid lab-fail moments shared by past guests across multiple seasons. You’ll hear stories involving exothermic sodium hydroxide reactions, PCR screening disasters caused by a simple reagent mix-up, forgotten spike-ins during RNA-seq prep, accidental sequencing of human DNA instead of plant viruses, and even being locked inside a cold room on the first week in a new lab. Along the way, the episode highlights the science behind each mistake and the importance of lab safety while reinforcing an important message for scientists at every stage: failure is part of the process, and every mistake becomes a lesson worth remembering. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.
Transcribe →Almost a virus – how AAV powers modern gene therapy
Mar 1800:32:59Tap to summarizeViruses are not always something to fear; they can also be tools to heal. This episode looks into this positive side of viral biology.Dr. Dan Mitchell, Senior Director of Analytical Development and Quality Control at Matica Biotechnology, joins the show to unpack the science and strategy behind viral vector manufacturing in a CDMO environment. He explains what CDMOs do, how they support cell and gene therapy programs at every stage, and why AAV has become such a powerful delivery vehicle. Dan dives deep into analytical development, describing how technologies like qPCR and digital PCR are used to quantify viral genomes, assess empty-to-full ratios, detect residual host cell DNA, and identify rare recombination events. He also discusses how sequencing, infectivity assays, and orthogonal analytics come together to ensure safety, potency, and regulatory readiness.In Lisa’s Career Corner, Dan traces his path from marine biology curiosity to high-containment virology labs, pressure suits included. He encourages young scientists to get into the lab early, embrace failure as learning, stay curious and find the patience it takes to succeed in science. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.
Transcribe →How sequencing and PCR work together in oncology research
Mar 400:33:08Tap to summarizeIn this episode of Absolute Gene-ius, the hosts dive into the evolving world of precision oncology, where detecting rare molecular signals can make all the difference. Dr. Kathie Sollweck brings deep expertise from her work in medical affairs and oncology to explain how modern molecular tools are reshaping cancer research. She breaks down liquid biopsy approaches, including cell-free DNA and circulating tumor cells, and explains why sensitivity and pre-analytics are critical when working with low-abundance targets. Kathie also clarifies how sequencing acts as a wide-angle discovery tool, while digital PCR serves as a laser-focused method for ultra-sensitive monitoring of known mutations, therapy resistance, and minimal residual disease (MRD). Together, these technologies enable more personalized, precise, and less invasive cancer researh strategies. The episode wraps with a fun and refreshingly honest Career Corner, where Kathie shares her non-linear journey from an aspiring actress to botanist to oncology expert. Her advice? Don’t fear sales roles, stay open to unexpected opportunities, and let curiosity guide your career because you never know where it might take you. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.
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When PCR gets dirty - tackling PCR inhibitors
Feb 1800:11:47Tap to summarizePCR inhibitors are everywhere and they can definitely mess with your data. From muddy lake water to complex viral vector preparations, inhibition is a reality that can’t be ignored.In this inaugural Absolute Gene-ius: Science Snapshot, hosts Jordan Ruggieri and Lisa Crawford revisit standout moments from past seasons to explore how scientists across disciplines deal with PCR inhibition. Through clips from experts working in wastewater surveillance, environmental microbiology, and gene therapy manufacturing, the episode highlights why inhibitors distort qPCR results and how sample preparation alone doesn’t always solve the problem. Guests including Ray Ketchum, Sarah Philo, Patrick Hanington, Dave Bauer, Kimberly Gomez, and Min Jin Kim explain both the biological sources of inhibition and the technical reasons digital PCR is more resilient. Together, these perspectives paint a clear picture of why dPCR is becoming the method of choice when overcoming inhibitors is required. Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the science we explore. Discover the Applied Biosystems QuantStudio Digital and Real-Time PCR Systems powering real-world research across neuroscience, oncology, agriculture, and more.
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