Rav Nachman identifies two types of fat on the stomach: bar chimtza, which can effectively seal a perforation, and chimtza, which cannot. To clarify which specific fat constitutes bar chimtza, the Gemara cites an independent statement by Rav Nachman regarding a halakhic dispute between the Sages of the Land of Israel and those of the Diaspora.

Rabban Shimon ben Gamliel rules that if internal mucus seals a perforation in the intestines, the animal is not a treifa. Rabbi Yochanan is cited as ruling in accordance with Rabban Shimon ben Gamliel on this issue and on an unrelated matter regarding mourning - that if a mourner returns home toward the end of shiva, they may conclude their mourning period alongside the other family members, provided they were not far away when the death occured. A discussion follows regarding whether the final halakha follows Rabban Shimon ben Gamliel on one or both of these issues.

To determine whether a perforation occurred before or after the shechita, a practical test can be performed by creating a comparable post-mortem puncture to observe and compare the tissue's reaction. Different sages note that this testing method is applicable to the intestines, lung, and windpipe; however, some outline specific limitations to this procedure.

The Mishna distinguishes between the laws of the keres hapnimi (inner stomach), where a perforation of any size renders the animal a treifa, and the keres hachitzona (outer stomach), which requires a rupture across the majority of its area to disqualify the animal. The Gemara presents seven distinct interpretations to identify precisely which anatomical section constitutes the inner stomach.

Rabbi Yehuda's dissenting opinion regarding the outer stomach is explained to mean that a perforation of either a tefach (handbreadth) or a majority of the area will render the animal a treifa.