Rav Bibi bar Abaye rules that although a standard hole in the windpipe requires the size of an issar (a coin), its clawing measurement (of redness) is a minimal amount, because the predator's venom burns and consumes the tissue continuously. Regarding the scope of the inspection required due to concern for venom, Rav Nachman testifies in the name of Rav that one must inspect from the base of the brain to the thigh, and not only adjacent to the intestines (as some hold). In an interesting story, Rabbi Yochanan defends the supreme authority of Rav against the queries of Reish Lakish, and consequently, Reish Lakish praises a different tradition of Rav, according to which an animal whose organs (simanim) were dislocated and was subsequently slaughtered is kosher.

It was established that new cases of treifot may not be added beyond those enumerated by the Sages, even if these injuries cause the death of the animal.

The Mishna enumerates structural defects that leave the animal kosher, including a windpipe that was perforated less than the size of an Italian issar, a brain membrane that was not punctured, and a liver of which a remains. This list gives rise to a fundamental dispute between Rabbi Yochanan and Reish Lakish regarding the scope of the lists in this chapter, which affects their approach to the ruling of Rav Matna, who rules that a femur bone that dislocated is a treifa. Rabbi Yochanan declares the animal kosher because the case was omitted from the exclusive list of treifot, while Reish Lakish rules it a treifa because it is absent from the exclusive list of kosher defects.

For the Babylonians, they defined that the size of the isser is similar to a Kurdish dinar, and the Gemara brings a story following this involving Rabbi Yochanan who went to a moneychanger looking for this coin,and told the moneychanger that craftsmen engaged in their work are legally exempt from standing before Torah scholars. How does this differ from craftsmen who would stand up and greet those Jews who were bringing their first fruits to the Temple?

Rav Nachman said that "up to an issar" it is not a treifa means up to but not including that size. Rava brings a series of difficulties against his view, but Rav Nachman answers them.