The Gemara brings a third source to attempt to prove Rav Yosef's assertion that Sumchos holds one who eats two olive-bulks of forbidden fat (chelev) in a single lapse of awareness is liable for two sin-offerings, but this attempt also fails.

A Mishna details four times during the year - the eves of the last day of Sukkot, the first day of Pesach, Shavuot, and Rosh Hashanah - when an animal seller must inform the buyer if its parent or offspring were sold that day for slaughter, due to high meat demand raising concerns of same-day slaughter. On these four occasions, the Sages also instituted that the purchase is acquired by the buyer upon the payment of money, and they compel the butcher to slaughter in order to supply meat for the festival eve. The Gemara explains this ruling either as an acquisition made on the buyer's behalf through a third party (zicha) without the buyer's knowledge, or as a restoration of biblical law on these days where payment alone transfers ownership (ma'ot konot), overriding the standard rabbinic decree requiring physical pulling (meshicha).

The Mishna and Gemara establish that the phrase "one day" (yom echad) regarding the prohibition of oto v'et beno is calculated according to the order of Creation (as those same words appear there), where night precedes day, unlike consecrated offerings (kodashim) where day precedes night. Rebbi derives from these words the obligation to give notification on these four festival eves.

The sixth chapter deals with the mitzva of "Kisui HaDam," covering the blood and opens with a Mishna stating that the commandment to cover blood applies to non-sacred (chullin) undomesticated animals and birds, but not to kodashim. The Gemara clarifies why kodashim are exempt from blood-covering and establishes the exemption as referring to birds consecrated for Temple maintenance (bedek habayit).