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Hey everybody, this is Hunter Williams. I hope you're doing amazing wherever you might be in the world. Today's video is going to be the TB500 masterclass. I actually wanted to do this one before BPC157 because I think TB500 is actually a little bit underserved. We all talk about BPC157 and rightfully so. I love BPC, don't get me wrong, but TB500 a lot of times is kind of always taking a backseat to bpc. And so today I really want to do a deep dive on what one. Just practically speaking, how do we approach the use of TB500 but also to highlight some of the things it's doing differently. Because a lot of times we just hear Wolverine blend and we associate BPC and TB500 when we just automatically think healing. And there's so much more that TB500 is doing and mechanistically it's a little bit different than BPC. We'll talk about the synergies between those two, but I'm really excited for this one. I love TB500 and as I've been along my own peptide journey, I've really come to have a soft spot, I guess for TP 500 or just just a really renewed appreciation for some of the amazing things it's doing, just beyond joint healing or injury healing. So that's what we're going to talk about today. As always, thank you guys so much for supporting me. As always, make sure you are on the email list. That is the best place to Stay in touch with me. And also too, if you want to get more direct access to me, check out the Axion Collective. The link will that link for that will always be on that one link that I have. That's kind of like my version of a link tree. It's you can check out. And then also too, I have a new thing that's like an AI chat. So I built that myself. What it is, it's not like chat, GPT or grok. It really is just a tool that analyzes all of the data that I have fed into it, which is just my personal video library of all the videos I have ever made. So it basically just draws on that. So think of it as a chat GPT version of talking with my video database, if you will. I also have a video database that you can go look through, you can search through and it will search the transcripts or anything. It'll take you to the video, all the old videos that I've made. But the chat is pretty cool because it basically just answers any questions you have. So if you ever think, hey, I want to ask Hunter this that he talked about in the video, the chat tool is the best place to do that. So without further ado, I'm going to share my screen and today we are going to go over the TB500 masterclass. All right, let's get into it. Today is going to be the user's Guide Masterclass to TB500. So a lot of people say, Does TB500 work? I understand that, but the right question is, what is TB500 and is it the same thing as Thymus and Beta 4? So what I want to explain first before we get into this whole thing is what TB500 actually is. Because I think there's a lot of confusion around Thymus and beta 4 and TB 500. So I really want to address that for you guys. So conceptually you understand what is going on there and then why you would use TB500 or why you would maybe use Thymus and Beta 4. So we look at Thymus and Beta 4. This is what is endogenous to the human body. So this is a natural full length protein found in every single cell or almost every cell in the human body. It's 43amino acids long. It weighs 4,921 Daltons and was first isolated from cath tissue in 1981 by a guy named Alan Goldstein at the National Institute of Health. Go figure. You know what's interesting about the National Institute of Health as a side Note, I promise this will be the only time I talk about GLPs today, I think. And all the GLPs came out of the National Institute of Health. Now they were commercialized, run through trials and everything by these larger pharmaceutical companies like Eli Lilly. But it is kind of interesting that these things are coming out of taxpayer funded institutions. But anyway, I digress. And the majority of published literature, clinically speaking, is around thymus and beta 4, not specifically TB500. Now let's look at thymus and beta, or excuse me, TB500 versus thyus and beta 4. TB500 is the synthetic peptide fragment built around the active LQ or LKK TetQ region, which is just seven amino acids long. So it's of that 43amino acid fragment. TB500 is the segment of 17 through 23 that it's seven amino acids long. And this is most of the time when you're purchasing from a research peptide company, what you are getting. And when we look at clinical literature, not a lot of it is around TB500 specifically, although a lot of it could probably translate. But anyway, there's a lot of confusion because most people will sell TB500 but they'll label it either as TB4 or TB500. And so this is why third party testing is important, or just knowing your supplier is important to know what you're getting because sometimes you might be getting either one. Now in most cases I don't think you're gonna have an issue with those, but we do want to understand that there is a difference. And sometimes the, the difference can play out in how we're using them practically. So when we look at peptide, obviously they are signaling molecules. So kind of like a software program that we're interesting the body to achieve a certain outcome. So we have insulin, we know oxytocin, we know growth hormone peptides, all those good things. TB500 operates the same way. And specifically when we look at the signaling of TB500, what it's doing is binding to G actin with high specificity and triggering a cascade of downstream effects centered on cell migration, tissue repair and inflammation modulation. That's why people love it so much. That's why it does so many amazing things.
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Let's go. Now, let's look first at this Acton system, because this is what really distinguishes TB 500 versus something like BPC 157. And I think when I was preparing these, I really wanted to draw the distinction of why TB500 is different and why you actually might practically get very different results when just using TB500 in isolation. So every cell in our body has a cytoskeleton, which is internal scaffolding that gives the cell its shape and enables movement. So again, think scaffolding. I've got the picture of the scaffolding right there. Think of a scaffolding as something outside of a building when you're building it that the workers are kind of working on top of in order to support themselves to finish the building. So the main building block of that scaffolding is something called actin. And G actin is the free monomer. Think of those as loose bricks. And F actin is the polymerized filament, which is the actual assembled wall. So when a cell needs to migrate toward an injury site, it converts G actin into F actin into bacteria to build new scaffolding in the direction of travel. Again, TB500 really works on this idea of cell migration, of going to where something is needed in order to repair. So actin makes up as much as 10% of all cellular protein, thymus and beta 4. And by extension, TB500 binds to G actin and keeps a ready pool available for rapid mobilization when the cell needs to move. So that's what we're dealing with in principle when we talk about TB500. So what happens when we inject TB500? We have G actin, which are basically the bricks. So these are the raw building material sitting at the site. They're available everywhere in the body, but they don't have direction to tell them where to go. So there's no coordination of what to do with G actin. So cells always have actin, but idle actin does not build anything. So it's just kind of the bricks laying there. Now when we look at the cell machinery, the workers are ready, capable and motivated. But without a foreman coordinating for the bricks to go somewhere, walls go up slowly and in the wrong direction. So there's kind of chaos on the job site, so to speak, for anyone that's ever worked on a job site. I grew up working on job sites for a large chunk of my life. You know that sometimes there's chaos on a job site, whether you're building a house, commercial building, or whatever. So TB500, think of this as the foreman. So TB500 binds to G Acton, which are the bricks, and releases it to the right workers at the right time in the right place. So TB500 is the manager that's coming in saying, okay, we've got the bricks over here, we've got the injury over here. Let's move these bricks to the injury to rebuild that specific injury. So the walls go up faster, straighter and the exactly the direction the injury site needs. So when we look at TB500, really what it's doing is orchestrating where the body needs to heal itself. And that's the beauty of peptides, right? Is the body can heal itself, but peptides are inducing a state or inducing a chemical signal that is actually allowing the body to do that in a much more efficient manner than it would. Because Again, we have TB500 in the form of Thymus and Beta 4 already in the body. We can use it Exogen to create this effect. So let's look at the the mechanism now that are actually driving tissue repair. So we have cell migration. This provides the actin supply that lets cells extend the leading edges and move efficiently to the injury sites that we're that we're experiencing. The primary driver of TB500's healing effect is this cell migration. Secondarily we have angiogenesis. So endothelial cells, which are blood vessel cells, require actin to migrate and form new blood vessels. So the sequence of TB500 specifically promotes formation of new vascular networks in healing tissue, which again is going to expedite healing. It's also anti inflammatory, it reduces pro inflammatory cytokines, su suppresses NF kappa B activation and decre decreases oxidative stress markers. It also modulates the inflammatory phase rather than blocking it entirely. I will say when we look at just using BPC for healing and then you combine it with TB500, my understanding is that BPC has much less direct anti inflammatory benefit. There is more indirect Anti inflammatory benefit. But TB500, I'd say even less so than probably KPV because that's such a potent anti inflammatory. But TB500 does actually directly suppress NF Kappa B activation and decreases oxidative stress markers. And then so when we look at the the inflammation aspect of injury, TB500 pairs well nicely with BPC because it's directly bringing down inflammation. It's also anti apoptotic. It activates the AKT survival pathway through integrin linked kinase helping damaged cells to survive the repair process rather than triggering premature cell death. Which I'll talk about a little bit later when we address this idea of theoretical concerns with cancer. It's also an anti fibrotic so it reduces scar formation by modulating collagen organization and suppressing myofibroblast activation. It also down regulates ROCK one and cardiac tissue specifically. I think for people that are dealing with heart disease or even after a heart attack, TB500 is one of the things I would immediately administer to those people because of its nature of being anti fibrotic. Also works on something called I'm going to pronounce do this, do my best to pronounce this. Oligodendrogenesis which promotes the differentiation of oligodendrocyte progenitor cells into myelin producing cells. This was demonstrated in thymus and beta 4, not necessarily the fragment of TB500. Now let's look at BPC and TB500. When we look at BPC, it's a growth factor signaling molecule and works on nitric oxide modulation. The best used is when it's injected near or at the injury promotes local tissue proliferation and growth factor cascade at the inj, the injection site which would be the injury site in this case. It also grows new tissue locally and the on site construction crew doing the actual building work. So if we look at BPC157 that's actually more of the worker, whereas TB500 is more of the foreman. We have TB500. It's a cytoskeletal regulation tool and actin driven cell migration tool. It distributes systemically regardless of injection site and it's effective even when the injury location is difficult to target directly. I will say when you look at these, obviously you could use them in a blend where you inject them in the same site. Typically if you have very systemic issues, you could inject TB500 just into your fat tissue in the belly and that's going to usually work in the areas that's needed most. For instance let's say your feet hurt and your hands hurt. If you inject TB 500 into your belly, you will get healing at both places. Whereas BPC157 inject your belly probably will have a little bit of effect, but you're going to want to inject that to the injury sites. Now you can inject TB500 the injury site obviously, but in the case of systemic inflammation, TB500 is going to have much more of effect systemically. For chronic tendinopathy or major injury, the combination is measurably better than either alone. And again, that's why we call it the wolverine stack. We get this one plus one equals three effect. Now when we look at TB500, let's talk about it in isolation. Who it's actually for one, you could have the chronic soft tissue injured athlete, a nagging tendon, tendon impingement or ligament issue that has not resolved a structured conservative care for over three months. This is the core use case and I would say you're probably going to want to do the dosing higher than you would if you were combining it with BPC1 7. We could have someone post surgery. Obviously this would be a great thing to use post surgery if you did have an injury that you were trying to heal. I think for aging athletes this works really well to just run periodically to help with recovery. And so for people that have had accumulated wear and tear from years of training with multiple small injuries that individually do not warrant surgery but collectively limit quality of movement training capacity, TB500 can be a miracle. And then also too again those things that we don't necessarily associate with it. I think the post illness recovery phase for someone recovering from significant illnesses or extended physical stress, the systemic migration and anti inflammatory effects have application beyond musculoskeletal repair. And, and when we look at that, I love TB500 obviously for injury, but for someone that had long Covid or some of these weirder diseases, chronic fatigue, Lyme disease, Ms. fibromyalgia, some of those things, TB500 does really well for a lot of those people. BPC2 but who should skip TB500? Obviously if you're a water tested athlete it is banned so I can't tell you to go out and take it. And for people with active or recent cancer, I think it's probably the best thing not to use angiogenesis. If we have active or recent cancer. Do they suppress or enhance cancer cells? We don't really know at this point but I think there's other things that you could use to improve the environment in the body. Before I would use TB500 and even in the form of something like Carax or kpv, I think you'd use those for injuries if you were someone that had cancer or recent cancer but didn't want to to use the typical healing peptides. Now let's talk about dosing because obviously this is kind of where everyone gets a little confused. So this is not formal in any sense. This is just from my own personal experiments and experience. And so I have a tier one dosing which would just be general optimization. This I would call 500 micrograms to 1 milligram sub Q daily. What this does, it maintains a steady tissue repair signal rather than pulse and clear. And I like to cycle this eight weeks on, four to eight weeks off. We look at this, this lower dosing, this is typically one which you will pair it with BPC with. So if you were using a blend of BPC and TP500 in a one to one ratio, which is what I recommend, I like the 500 mics to 1 milligram range for that. And this is just going to be background. So if you have a serious injury, I would not say that it's going to be something that will heal it right away. Over time it could probably help, but I like that dose. For people that just want better recovery from their workouts, maybe you're in a muscle building phase. I always recommend people that are attempting to put on muscle actively to use BPC and TB501 because BBC enhances the response to growth hormone or growth hormone peptides and also because they're going to help with muscle recovery. We have the tier 2 dosing and this is where I'm going to say active injury and recovery. What this would be is 2 to 2.5mg sub Q twice per week for four to six weeks. And then you could go to 2mg once per week for four to six weeks for maintenance and then the total cycle would be eight to 12 weeks. But you're going to be doing a higher dose less frequently to help with active recovery. And for someone that's injured, I like getting up into the higher dosing for things that are more acute and then for someone severe or complex injury, 5mg twice per week for the first two weeks and then 2mil 2.5mg twice per week for four weeks. Then 2mg once per week for four to six weeks maintenance and the total cycle would be 10 to 12 weeks. When we look at the tier 2 and tier 3 dosing, what I like to recommend to people is that you use it until you are 95 to 100% healed. And I worry much less about staying on for only 8 weeks or only 12 weeks. If I have an active injury, maybe it takes 16 weeks, maybe it takes 20 or 24 weeks. That's okay because it's much better to have those agents in the body to heal them rather than to potentially put myself at risk for more injury. And so for some of those more severe ones, that's what I do. For instance, like if I were to pull a muscle today, later when I lift weights, if I was going to use TB500, what I would do is inject like 2.5 milligrams or maybe even 5 milligrams if it's really severe. And I would do that a couple days a week until that muscle heals up. I wouldn't really go with the lower dose. I know I would go with the higher dose. But again, if I was just doing it in the background for an improvement in recovery and everything, I would do that.05 milligrams to one milligram dose. And so again, there's no gospel, there's no, there's no set law when it comes to dosing. But that's kind of how I approach the dosing and what I would use it for. Again, just to sum up, there's the chart. So we have general longevity. That's the tier one. We have the acute soft tissue injury, tier two, chronic tendinopathy, tier two, post surgical, post surgical recovery, tier two and then pre surgical conditioning could do tier two. I would usually stop seven to 10 days prior and then restart within seven to 14 days after the surgery. Now let's set some expectations. When we actually notice something, it is not one that you necessarily feel acutely. You're not gonna inject TB 530 minutes later, have this massive epiphany or anything. Or maybe you will. I never have though. Cellular signaling usually begins within 48 to 72 hours after the first injection. But observable improvement depends entirely on the tissue type being repaired. Again, this is where everyone is so different. Tender slow muscle is typically faster and we wanna manage expectations. When we look at 48 to 72 hours, cellular signaling is initiated. We have G actin binding beginning and no subjective change is usually there. Then we have 10 to 14 days. This is the first window of improvement in acute muscle strains that you'll usually see and Then inflammation and stiffness may begin to ease. Two to four weeks in, this will usually be where you have chronic inflammation and joint stiffness start to improve. Four to eight weeks is where we'll see more of the tendon and ligament repair window. So torn Achilles, things like that, that's usually where you'll start to notice things moving in the right direction tends to heal slowly even with optimal intervention. And then three to six weeks is usually the window where if you are post surgical that you will start to do things and see, see things moving the needle. So again, everyone's going to be different. But I do like to tamper expectations because you know, if you hurt your back, I was actually talking to someone about this yesterday, you hurt your back, don't take two injections, I think it's going to be healed. If it's a severe injury, it's going to take a while to do so. So just know that the cumulative dosing is usually where you'll see the den. The, the benefits. When we talk about stacking these, like I said earlier, we have a one to one ratio of TB 500 and BPC 157. And so I like 250 to 500 micrograms of each every day. And again, this is easy because most of the vials that come mixed together, they're in a one to one ratio. And then if you did have those, I would do at least 500 micrograms at least two times per day just to get the benefits of both of those. Now let's look at cycling. I hinted at this a little bit earlier and typically it appears that TB 500 does not desensitize. There's no clear evidence that receptors down regulate with continuous use. However, that doesn't mean that it doesn't, it's just that we don't have evidence that it does. So the rationale for cycling is different here than it is for hormones or receptor binding compounds. So three practical reasons to cycle anyway. One, long term safety data is limited. Two, cost and injection burden are factors. So again, it could just be a cost thing. And then cycling creates natural durability assessment windows where you can observe what holds during the off period and make better decisions about the next cycle. And so again, I, I really like cycling for that reason. Now if it's a case of getting healed, I would say use it until you're healed. But if it's one of these things of hey, I just kind of want to recover better, I just kind of want to feel better, that is the classic case of when I would strongly recommend cycling. So you could do eight weeks on, four weeks off. That's the default pattern for a lot of people. Repeat two to three times per year as needed, 12 weeks on, four weeks off. Obviously that would be more involved if you would say that you're a little bit more of a severe case. And then you could also do a targeted short cycle of four to six weeks with some of those higher doses and see how you feel. But again, you have to use discretion when it comes to that. Just because we don't have evidence that the the receptor doesn't desensitize doesn't mean that it, it doesn't. We just haven't found anything definitively that is the case. Now, when we talk about reconstitution, the easiest thing is to add 2mls of water to a vial. So if you have a 10 milligram vial and you add two mls of water, that's going to be 5 milligrams per milliliter. If you have a 5 milligram vial, you got 2 mls of water, that's Going to be 2.5 milligrams per milliliter. And so you can see there on the screen, if you want to screenshot that just the reference. Obviously that's why I have the peptide cheat sheet. But very easy to mix. And I will say TB500 typically does not sting when you're doing it. I like sub Q injection also too. I prefer to rotate the sights. If you are doing the belly, just kind of think about your belly as the top half of a clock. You go around it, 9 to 3 is going to be where you want to rotate. Starting on one side and then kind of rotate around there and then 29 to 31 gauge insulin needle and then slowly inject. So same thing for most peptides. When we talk about blood work and improvements, it just does help. I always tell people, before you get your peptide cycle in line, just make sure that you are doing blood work to make sure that everything is okay, that you are a good candidate for it. When we look at other peptides, we talked a lot today about bpc. This is the Wolverine stack. I do like adding in GHK for the collagen remodeling. You could introduce one to two milligrams daily near the injury site after, after four weeks of the wolverine stack. I also really like a growth hormone peptide stack. So you could use CJC and ipamorelin. You could use tessellin. And I, you could use MK777 pick your poison. Not really poison, but just pick whatever you want to do when it comes to the growth hormone stack and there there usually is a synergistic effect when we talk about that. Again because of the growth hormone receptor sensitivity. Then also for the post illness recovery people with Thymos Alpha 1 works really well. You can do Thymos Alpha 1 the the recommended dose in most countries is 1.6 milligrams twice per week. But for people that are coming out of an autoimmune issue or some again one of those more weird issues, I recommend Thy Alpha one with it. But again when we talk about mixing compounds we don't want to throw too much in there. Obviously TB500 is part of the close stack, so you have BPC, GHK and KPV alongside of it. I think that's great too. Now let's talk about some troubleshooting. So the most common problems what to do? I would say one especially for people with injury, the most common thing is I don't feel anything. Now let's check. This is your baseline already good and there's less room to improve. In some cases you might not feel TB500. Your dose could be too low, so a lot of people are in that 500 microgram range dose. I will say if you don't feel anything, keep going up the dose. I think it's fine to take up to 5mg daily a couple times a week of TB500. I've done that before and I've responded really well to it. Your duration may be too short. Again, if you're just using it for four weeks, you may have to continue into eight, 12, 16 or even 24 weeks. And again, the source quality could be poor. Again, I'm not a huge person that wants to go around and scare everyone, but just make sure you're getting it from a reputable source.
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we look at injection site reactions. This could be something that people deal with. I would say with TB500 is much less than others, but still a thing. And again, you could have redness, itchiness, things like that usually Benadryl is the easiest thing to do. And in most cases that will help. Some people do get lightheadedness after injection. If this is the case for you, eating something beforehand could be beneficial. And then again, just make sure you have good quality product. But I will say of all things, TB 500 is usually one of the ones. Is TB 500 legal in the US? It's a regulatory gray zone, so it's not FDA approved. Hopefully it's one of the ones that gets moved on this category two list. I think the biggest question that people ask is will TB500 cause cancer? The theoretical concern comes from the angiogenic activity. A 2023 study found that thymosin beta 4 actually suppressed lung cancer via Jack2 and STAT3 pathway inhibition. Meaning that the relationship is more complex than simple angiogenesis causing tuner. And again, I'm not one to say like okay, we have this data here that shows a suppressed. Am I going to go around tell tell people that it suppresses cancer? No, because we just don't really know. But it seems like if it was at this point we would be saying where are the bodies? And again, for the healthy user, this is really more of a theoretical concern. Will it affect my hormones? No. TB500 is not bind to androgen, estrogen or progesterone receptors. It's not affect the HPG axis. So again, there is no hormone thing. Meaning that I think for a younger person, again I don't get into really talking about this in public, but for a younger person I would not worry about it affecting hormones. Cost wise you're probably usually running somewhere of, you know, 200 to 600 for a cycle. Can you take it orally? There are oral formulations. I will say TB500 has poor oral bioavailability. Maybe not zero, but sub Q injection seems to be the best route. I think when we look at the oral bioavailability, I don't know this too much, but it seems like some of those other fragments might be better for oral use rather than TB500, which is the 17 through 23. Does it grow hair? Animal studies show stimulation of hair follicle stem cells. No human trial data exists. These are more anecdotal. However, there are some hair topical products with some absorption enhancers that claim that it works. I've never used those. Fortunately for me, at this point in my life I don't have to worry about hair loss. If anything, I have too much hair. It grows rampant on my body. But I will say that there Are people out there that seem to have said that it does help with hair loss. Where does the future look like for TB500? The most active clinical programs are pursu pursuing full thymus and beta 4. I would say the full thymus and beta 4 is typically what you would want to use in more severe cases of heart failure or stroke or things of that nature. I would lean on that. We do have other fragments of Thymus and beta 4 like acsdkp. I've seen that being sold out there in the wild. Now this has specific anti fibrotic activity and meaningful better oral bioavailability. So if you did take the oral version, I would take that. ACS, DKP, inhaled recombinant human thymus and beta 4 demonstrated efficacy in pulmonary fibrosis models in a 2025 study. So interest testing there with that. I do know there's a ton of data for lung health with TB500 and in anecdotal reports for people that I help, TB500 has been massive at improving lung health. And again that's going to be that higher dosing like 2-5mg a few times per week to help with that. Again at the time of this, it's June 3, 2026. Looks like they're going to meet in July of 2026 and TB 500 is on the table there. Hopefully they get moved from category two to category one, which means that compounded pharmacies could make it and clinicians could prescribe it in that case, even though it would not have FDA approval. What do we know? TB500 has a very solid foundation when we look at the mechanism. Just to sum again, actin binding drives cell migration, angiogenesis and anti inflammatory signaling. Also anti apoptotic activity and anti fibrotic remodeling. When you think of TB500, think of this as the manager that is sending the things in the right direction. Dosing and cycling. We talked about the doses and cycles and again works really well with the PPC, TB500, VPC, KPV, GHK, those are all really good together and have synergistic effects. And remember, TB500 is just a tool. So when we look at miracle cures, this is not gonna be a miracle cure if you have degenerative disc disease. Is TB500 gonna help? I don't know, it's not gonna hurt. But again, just have expectations that are set in reality to know that can help. But in a lot of cases it's not gonna be A miracle cure for some people depending on the severity of the injury. And that is it for the slides and that is my masterclass for TB500. So in comparison to retatrutide and some of the other peptides we have, not so much that I have to extend this to an hour. Got this one nailed in around 30 minutes or so. But I do think TB500 is one of the most important peptides when we look at people with obviously injuries, soft tissue injuries, but then also people that have lung issues or some of those more chronic illnesses that can combine well nicely with Thymosin Alpha 1 and some of the some of the other immune peptides. I'm a huge fan of TB500. Honestly if I pulled a muscle in my back and I could only use BPC or I could only use TB500 I think I'd probably have to lean on TB500 because of the more direct anti inflammatory benefit. Obviously if I could use them both I would and obviously I could use Cardillacs, GHK&PEG, MGF and KPV, I would use those too. But if I could only use one out of BPC and TB500 I think I would probably lean on BP or TB500. Now if it was a gut health issue I would definitely lean on PPC 157. If it was strictly for muscle recovery purposes, just for an athlete I would probably lean on BPC157. But I think in the case of an injury I like TB 500 a little bit more. Obviously it benefits to combine them both. That's it for this one. Thank you guys so much. I would love to hear your feedback. It seems like these master classes are getting a lot of good feedback and I will definitely continue to do them as everything moves short form, like I've said, I am moving long form and to be as boring as possible. I know it's not really boring for you guys but in terms of the way content is made, I want my content to be much more educational, fact based and really, for lack of a better way to say it, kind of dry because I love these. I think people learn by immersion and when you present it in this way, hopefully it's helpful for you guys to where you really have a solid footing of what TB500 is and kind of just the most condensed version to give you the most amount of information so that you can go out and whether it's in your own research, whether you're a clinician or, or whether you're just helping people and coaching people you know how to implement this in your practice to help people get the results that you want to. So in closing thank you guys so much. It is truly a dream come true that I get to do these videos and that it helps anybody in the world and now that it helps so many people man it is just some days I'm like pinch me because this is so awesome that I get to do this. So whatever form or fashion it is that you support me, whether it's using my code at places, being on the email list, being in my private group or or just sharing this with your friends and family, you have no idea in how far that goes and helping me bring these messages to you. So as always in closing thank you guys so much. Love to hear your thoughts and feedback in the comments of wherever you're watching this and I will see you in the next one. Peace.
Episode: The Complete TB-500 Masterclass: Dosing, Stacking, and More
Host: Hunter Williams
Date: June 5, 2026
Hunter Williams delivers an in-depth "masterclass" on TB-500, a peptide often overshadowed by its more famous counterpart, BPC-157. This episode seeks to demystify TB-500 by exploring its unique mechanisms, practical use cases, dosing regimens, stacking strategies, and troubleshooting tips for optimization-minded listeners. The goal is to provide listeners—from clinicians to biohackers—with actionable insights for leveraging TB-500 effectively.
“TB500 a lot of times is kind of always taking a backseat to BPC…there’s so much more that TB500 is doing and mechanistically it’s a little bit different than BPC.”
– Hunter Williams [01:09]
“When we inject TB500…TB500 is the manager that’s coming in saying, ‘okay, we’ve got the bricks over here, we’ve got the injury over here. Let’s move these bricks to the injury to rebuild that specific injury.’”
– Hunter Williams [08:50]
Indications:
Contraindications:
Tier 1 — General Optimization:
Tier 2 — Injury & Recovery:
Tier 3 — Severe Injury:
“If I pulled a muscle today…what I would do is inject 2.5 milligrams or maybe even 5 milligrams if it's really severe…I wouldn't really go with lower dose.”
– Hunter Williams [17:50]
“Cellular signaling usually begins within 48 to 72 hours after the first injection, but observable improvement depends entirely on the tissue type being repaired.”
– Hunter Williams [20:45]
“When we inject TB500…TB500 is the manager that’s coming in saying, ‘okay, we’ve got the bricks over here, we’ve got the injury over here. Let’s move these bricks to the injury to rebuild that specific injury.’”
– Hunter Williams [08:50]
“One plus one equals three…that’s why we call it the Wolverine stack.”
– Hunter Williams [12:30]
“For someone that had long Covid or some of these weirder diseases, chronic fatigue, Lyme disease, MS, fibromyalgia…TB500 does really well for a lot of those people.”
– Hunter Williams [14:40]
“If I could only use one out of BPC and TB500, I think I would probably lean on TB500 because of the more direct anti-inflammatory benefit.”
– Hunter Williams [30:47]
For a quick reference, here are key time markers:
Closing Note:
Hunter’s candid, fact-based, and actionable approach makes this masterclass a foundational listen/guide for anyone interested in leveraging TB-500 for injury, recovery, or overall health optimization.