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Learn more@windows.com studentoffer while supplies last ends June 30th terms at aka mscollegepc. Hey everybody, this is Hunter Williams. I hope you're doing amazing wherever you might be in the world. Today's video is going to be the KPV Masterclass. I am really excited for this one. I always get excited for these, but I think specifically about kpv, I'm really excited for a couple reasons. I think one, the biggest one is that I think KPV is so underrated when we talk about peptides. Obviously there's, there's all the peptides that I love, right? BPC, TB 500, you know, GLPs, all those out there. But KPV is so underrated. I think the amount of people that know about KPV or at least know how to intelligently use KPV is so much fewer than there's actually out there that would benefit from this. So I'm excited for this one because I think there's probably a gap in the marketplace just of what people understand about KPV and actually using it. The reason I know is because having, you know, just seeing the commercial side of the peptide market, this is like one of the least demanded, but in my opinion it should be the most demanded peptides out there. And so I'm not saying that to try to push KP by any means or anything like that, but I think it's one that is not as understood in terms of how to use it and what actually it can do because it's so versatile and it do does so many things. Obviously you probably have heard of the CLO blend. KPV is a part of the CLO blend, but by itself it really can stand on its own for some of the things that it does. So that's why I'm so excited because I think this is one of those ones. Everybody knows about GLPs, everyone knows about BPC, TB500, you name it. When it comes to some of the peptides that people don't know that much about, I would put KPV in my top five peptides all day, every day. And I think a lot of people don't even realize what it does or how to use it. So that's why I'm so excited. But we're going to cover that today. We're going to go over everything that I could have possibly thought about from how to use so that by the end of this video, however long it ends up being, you have a very good understanding of what KPV does and how you can actually practically research with it. Before I do that, as always, just make sure you are the on the email list that is the best place to stay in touch with me. The link will be in the bottom of the description of wherever this video is and also too if you want direct access to me, the best place to do that is the Axion Collective. We do live coaching calls every Thursday night at 8pm Eastern in there. We've got over, I think like 250 people in there now. It is awesome. We have some amazing people in there, some amazing coaches, amazing doctors. Uh, so it's a really good hub place where if you want to ask questions about peptides, you can obviously privately message me, message me but then also talk to some of the amazing people in there as well. And then last thing, just because I recently developed this, check out the AI chat tool I have. It's just chat.hunter williams health.com seems to be working really well. A lot of the people that want to get questions answered from me but I don't have time to just because I don't have the bandwidth and day just to answer questions for people that are not in my private group. Make sure you check out that tool because it seems to to be really well. I put a lot of time and effort into building that and I've gotten really good feedback on it so far. So check that out and play around with it if you want. But without further ado, I'm going to share my screen and today we are going to go over kpv. All right, let's dive into it. Today we're going to cover kpv. We're going to Have a complete user's guide, slash, masterclass, whatever you want to call it. What we're going to cover is what KPV is, how it works, who benefits. And that's what I said in the intro is there's so many use cases for kpv, I think it's, it's vastly underutilized in the peptide space right now. Some dosing protocol. Luckily the dosing protocols are very straightforward practical use. Also, how to stack it with other peptides and then just what to track and what to measure objectively and subjectively when you are using it. Now what is kpv? It's pretty cool. It comes out of this melanocyte stimulating hormone family. It just has three amino acids, one of which is lysine, the next is proline and the next is valine. And the single letter codes for those are KPV. Hence we have KPV. The molecular weight is under 400 Daltons. It's tiny by peptide standards. So again we look at peptides, it's very tiny relative to like GLPs which can be upwards of 50amino acids depending on the one. Like I said, it's a fragment of alpha melanocyte stimulating hormone. It is the C terminal tip of that molecule, residues 11 to 13. So think of KPV as the active tip of a much larger molecule, the part that carries the anti inflammatory punch. What's really cool is we have this hormone called alpha melanocyte stimulating hormone. This is where we have melanin one, melanitan two, PT141 and KPV. So all of those peptides are fragments of that total hormone. And what's pretty cool is we can isolate some of those fragments to have a specific effect. And so what we'll see is that kpv, it doesn't cause you to have tan or tanning, or it doesn't cause you to have erections like PT141 might. But it does have an anti inflammatory benefit. So basically we're harnessing anti inflammatory nature of that alpha melanocyte stimulating hormone and then applying it in a therapeutic application that we will cover today. This is pretty cool. The Discovery story so it goes back to 1989. James Lipton and Mary Hiltz at University of Texas Southwestern asked which part of alpha MSH carry the anti inflammatory activity. So they isolated the C terminal tripeptide and tested it in a mouse ear inflammation model. And they found that KPV alone was as potent as a high dose corticosteroid which was a landmark result. And again, when we go back to the therapeutic applications, how rampant is the use of corticosteroids today? I'm not saying that they, they shouldn't be used for the certain popular or certain cases that they're popularly used for. When we look at kpv, we could get so much out of it that doesn't have the side effects of the corticosteroids. And 30 years later we have that paper that found in the field and we have pre clinical research. Obviously this was on the category two list. We'll talk a little bit about that today. But let's look as from a system, system level, what it looks like. So we have this thing called NF kappa, which is a transcription factor. So it travels into the cell nucleus and switches on inflammatory genes. So think of NF kappa B. You probably hear that a lot on different podcasts and everything. Think about that as a master switch for inflammation. Basically it's going into the cell and saying, hey, genes we are going to express towards inflammation. So in chronic disease, the switch gets stuck on. And so whatever it is, whether it's autoimmune disease, whether it's an injury, things like that, it's going into the cell, switching on this inflammation and then it gets stuck. And so we're in this perpetual state of inflammation. We have inflammatory bowel disease, rheumatoid arthritis, chronic tendinopathies, eczema, psoriasis. All of these have this NF cap, the kappa B transcription factor turned on. And what KPV does, especially that BPC and TB500 don't directly do, is it blocks enough kappa B. At the nuclear entry step, the alarm tries to fire and gets shut off at the door. So think of KPV as blocking this NF kappa B from being able to get into the cell. And you see the little diagram with the NF kappa B switch off, we have no inflammation. When the NF kappa B switch is stuck on, we have all these inflammatory issues that so many people seem to struggle with in the world today. For whatever reason, we don't have to get into that, but obviously it's, it's something that is rampant in our society today. Now let's look at the mechanisms of kpv. So KPB blocks enough kappa B at the nucleus. Enough kappa Bs active form, which is the p65 subunit, needs a transport protein called importin alpha 3 to enter the nucleus. What KPV does is it competitively binds to important alpha alpha 3 or excuse me alpha 3 at the exact site p65 uses. So KPV gets their first p65 cannot get in and inflammatory genes do not get switched on. So think of this as kind of saying, hey, nope, you see the the picture there? If you're watching the video, there's a door and it's locking this NF kappa B from getting in. So this is unusual for a very small peptide like KPV. Most anti inflammatories work upstream a la BPC and TB500. KPV works at the transcription factor itself, which is a more precise and direct intervention. And again to extrapolate that to practical use cases. This is why I feel like it's so powerful and so underutilized because people don't realize from an inflammation standpoint what it's doing to directly block this inflammation. Let's look at the mechanism 2. We have Pept1 mediated uptake. So KPV is a substrate for a transporter called Pept1 which normally pulls dietary peptides into intestinal cells. In a healthy colon. Pept1 expression is low. In an inflamed colon, particularly in someone with IBS or IBD, pepti1 gets upregulated. The inflammation itself increases the transporter that pulls KPV into the cell, which means that KPV concentrates exactly where it's needed. Most drugs would kill for this kind of targeting. And then again, if you look at the steps there, we have a healthy colonial low KPV uptake. Then the KPV dose is administered and you could do that orally or injected. And then we have an inflamed colon to which we have this pept1 expression. And then the KPV concentration, the disease site, we get targeted drug accumulation at the site there. Specifically in the case of the colon, which is pretty cool. Then we have mechan or not really mechanism three. But what it does not do, it does not activate melanocortin receptors like most of the other alpha MSH peptides. And despite coming from that, it does not activate those receptors. We don't get any sort of skin darkening. KPV and Melan2 share a parent molecule, but hit completely different targets. And there's also no appetite suppression. There's another peptide I forgot to mention called SET melanotide that is a fragment of alpha MSH that causes appetite suppression. We don't get any appetite changes, obviously, no libido shift, no flushing or anything like that. Again, a very clean mechanism with none of those negative side effects. With the other alpha MSH peptides. Zootopia 2 has come home to Disney Plus. Let's go get ready for a new case. We're gonna crack this case and prove we're victorious partners of all time. New friends. You are Gary the Snake and your last name the snake. Dream Team. Habitats Zootopia has a secret reptile population. You can watch the record breaking phenomenon at home. You're clearly working at Zootopia 2. Now available on Disney Plus. Rated PG. Now why is it unique relative to some of the other anti inflammatory peptides? It's the only commonly used peptide that directly blocks enough kappa B at the nucleus, not upstream. We also get smart tissue targeting. It concentrates in inflamed tissue through Pepti1 upregulation. Like we talked about, the disease state enhances the delivery. Just as a side note, what's pretty cool, I actually was reading a paper the other day. They've actually developed an oral version of KPV that is able to make it through the gut barrier to actually reach inflamed tissue in other parts of the body. Which is pretty cool because if you look at like bpc, even oral kpv healing the gut. We're not necessarily getting healing in our knees or our shoulders, wherever you might have an injury. But it does appear now, at least in rat studies that we have a way to modify the delivery of KPV to take it orally but actually reach the target tissue. However, we can always inject it as until that's available to use to reach the inflamed tissue. For instance, if you have a hurt shoulder, elbow, knee, something like that. We also have a very clean side effect profile. I will say KPV is one of the least side effect peptides or least side effect inducing peptides that I have ever seen. Maybe once or twice I've heard of people saying that they have a negative reaction to KPV in those cases. My guess is that that was a sourcing issue, not necessarily a KPB related issue. But for people with MCAS or people that are very sensitive, you guys all know these people. You might be one of them. Some of the people that I, I call the no disrespect towards them, they're just the nothing worked for me people. They have a reaction to almost everything. And a lot of cases I think that's, that's very real. I'm not saying it's a psychological thing. It's very real because their immune system is just basically being pushed to the brink. And I think KPV is One of the peptides that I would introduce to those people because it's so gentle, there's so small of a side effect profile and has such profound healing effects. And it's also one of the few peptides because of the size that has real oral bioavailability, which is rare and obviously clinically valuable. Now, I won't sit here and tell you that taking oral KPV is going to heal a torn meniscus or a torn labrum. However, it will help the gut, especially for these people that have oral issues. But I'm a fan, we'll talk about this a little bit later into the presentation. I'm a fan of doing both at the same time. A lot of people confuse KPV as something similar to BPC157, but they're completely different. And that's why KPV I think works energistically, but also is so unique. And honestly, like if I, if I were taking my, rather I would take KPV over bpc. Let's look at some of the people that do really well with kpv. The first one is going to be the IBD patient. These are people that have Crohn's disease, ulcerative colitis. KPV has the strongest preclinical case here. So pept1up regulation in inflamed colons means the peptide concentrates exactly where the disease live to be able to suppress that inflammation. Obviously oral dosing makes the most mechanistic sense for this population. But again, I'm a fan of taking the oral and injectable at the same time. But again, doesn't mean that it would be the only thing. There are other types of medications, bpc, lorazatide, other different peptides that also work really well to help heal the gut. But again, KPV would be the ones, one of the ones that I would lean on. We have leaky gut and ibs people that really get maybe not as far as Crohn's disease but have bloating food sensitivities, post infectious IBS gut symptoms that flare with stress. I, I think especially for the people that have severe food allergies or food sensitivities to like gluten or dairy. KPV can be a game changer for them. It addresses the inflammatory component which is often present but not the whole picture. And it's the best. You obviously get best results when combined with diet adjustment, stress management and then other peptides like BPC and then two. I think one of the, the Most underused cases that people fail to realize that works so well is, is for people that have plateaued. When it comes to a healing perspective on BPC and TB500, someone running BPC plus TB500 of a chronic tendon injury or stalled rehab often plateaus because inflammation is the rate limiting step. I think if, if I could drive home one point today about KPV is that when it comes to healing, inflammation is the rate limiting step. And we look at BPC and TP500, they rebuild tissue through nit, nitric oxide growth factor and remodeling pathways, but they do not directly, they indirectly, but they do not directly suppress inflammation. And so when we pair KPV with both of those, it breaks the plateau by addressing the inflammatory environment that prevents repair from sticking. And again, think of KPV if you're someone that's taken bpc and taken TB500, not going to say KPV will fix everything, but if you've really stalled out, maybe you got good progress with those and for whatever reason you just could not get the injury fixed or the healing issue fixed. KPV will often be something that can help you break through that plateau. We look at another ideal candidate. I love KPV and the use of autoimmune skin issue and MCAS patients. And so for autoimmune flares, rheumatoid arthritis, psoriatic arthritis and autoimmune skin conditions, all of those involve some sort of NF Kappa B dysregulation which is obviously KPV's primary target. And you can apply it topically as well. I haven't talked about that yet, but KPV topically in addition to the injection and oral works really well. Chronic skin conditions like eczema, psoriasis, rosacea, topical compounded cream plus oral or sub Q KPV for combined local and systemic effect works really well. And then lastly for mcas, which is mass cell activation syndrome, many practitioners report meaningful benefit and they like to start very low and titrate slowly obviously. Cuz that's usually a very sensitive population. But I've seen it seen to do really well in those people. Should anyone skip it? Well, I think this is one of the ones. Again I think that there's, there's almost a use case for everyone. But if your main problem is not inflammation, it's not gonna help with sleep apnea, thyroid dysfunction, hormone dysfunction, things like that. It's also not gonna give you like a performance edge. So I wouldn't take KPV Thinking that you're gonna like train harder or anything like that. It could help you recover better for sure and have a better gut, which will then help obviously with performance, but not directly. Again if diet, sleep and stress are under addressed, KPV will not fix those and it's not going to you. You might get a little bit of benefit but if your underlying foundational issues are not fixed, not gonna happen. Obviously avoid during pregnancy just because we don't know, we don't have any sort of data around that. And then separate KPV from antibiotics, ACE inhibitors or things like that because those can also work on peptide pept 1 competition. So at this point we just don't know some of those drugs that also work on Pepti1 uptake. Let's look at dosing. And so we have the, the tier one dose, I would say the lowest dose. And this is going to be. If you're just introducing someone, maybe they're more sensitive. I would say two 50 micrograms once daily. You could do this oral, in an oral capsule or subcutaneous and I would do this for four to six weeks. This is best for low grade chronic inflammation or general healing and longevity stacks. I would say this wouldn't even be. This would not be a bad idea. It would be a good idea if you just want to have some background anti inflammatory, not something that you necessarily need to heal. But sometimes I will just do this when I'm traveling if I'm exposed to non organic food that I know I might have a predisposition to have a little bit more sensitivity to. I love carry carrying KPV when I travel just because in instances like that it really helps my digestive system if I'm exposed to things or if you're traveling out of the country, maybe you're not used to the water. I was recently in Mexico speaking at a conference and I always carry KPV with me and my gut seems to do really fine anytime I'm drinking water or eating food that I'm not usually exposed to. And again that low dose is enough to nudge inflammatory tone toward or downward without committing to a heavy protocol and a good starting for starting point for people new to KPV or if you just want something in the background. Tier 2 is going to be active healing and this is going to be 500 micrograms once daily sub Q or 500 micrograms twice daily oral. I would do this for at least six to eight weeks and this is going to be best for active gut healing, chronic injury Mid to moderate autoimmune and most skin conditions. And, and this I would say is like the average dose of kpb. And so this is gonna be, if you're running a cycle where most people will lie, Tier 3 is going to be the severe inflammatory presentations. I would say 1,000 micrograms per day. You could split this up into two doses, one in the morning and one in the evening. And in my case, when I've used this for injuries, it seems to work really well to take it once in the morning. For instance, I had unfortunately a really bad reaction to testosterone that I injected once upon a time and I had this huge swollen knot and I would take kpv. In the morning, I injected KPV more near it and it would work great. But by the afternoon or the evening the pain would start to come back and I would inject KPV again and kind of, kind of almost act like an ibuprofen in a way to just help relieve some of the pain and suppress some of the inflammation. And for anyone that's ever just gotten a bad reaction to injections, you know how painful that can be when you have a lump and everything. And so I would love KPV for that case, but that would tell you where I would be. Where you have this more severe inflammatory, inflammatory presentation. You could go up to 1 milligram per day. I've gone higher than 1 milligram per day. And honestly the difference between 2 and 3 milligrams versus 1 milligram doesn't seem to have that much more of effect. Meaning that I think 1 milligram for a lot of people. I'm not saying that you can't do it. You can obviously do whatever you want. I think beyond 1 milligram per day it's probably just unnecessary. Meaning that you can only block NF kappa B and have so much of an anti inflammatory effect. It's kind of like a completely different analogy. But if we look at something like ibuprofen, 800 milligrams versus 1600 milligrams of ibuprofen, how much is that really gonna make a difference? I don't think that much. Usually 800 milligrams is kind of the, the big dose to which someone will really say like, okay, I'm feeling it and I don't need to go beyond that. I think it's very similar in principle to KPV beyond 1 milligram. I just don't see in my own experience and other people that I've worked With that much more benefit of going above and beyond one milligram per day. And again, even if you're, you're, you're new to it, you could start low and then go up to here. But uh, I think that's great. And you can obviously do this in oral as well. So you could combine another 1000 micrograms orally per day to help alongside that. And again, best for IBD flares, severe mcas and post surgical inflammation. And again for this level, I would say two to four weeks and then you could go back down to the 500 micrograms per day range. When we look at dosing by purpose, let's talk about gut healing first. The oral kpv dose is 500 micrograms twice daily for six to eight weeks. Pairs very well with oral BPC at 500 micrograms twice daily as well. This would be for ulcerative colitis, Crohn's, ibs, leaky gut, food sensitivities and post antibiotic gut damage. I will take an aside briefly and just say as someone that has struggled with post antibiotic gut damage when I was younger, KPV could be a miracle cure. I just wish when I was between the ages of like 17 to 21 that I had KPV because I was on heavy antibiotics as a teenager for my acne and that ruined my gut. And I had all of these gut issues for at least six or seven years after taking that as a teenager. And for someone that does have to use antibiotics for whatever reason, I love KPV Now I wish I would have KPV in two because it would have helped with my acne. So I wouldn't have had to be on antibiotics in the first place. I could have just used KPV and that probably would have helped a lot with my acne. Even when I have acne flare ups as an adult here and there, KPV works really well for that. And then we also look at the mechanism when we combine it with bpc. BPC drives endothelial repair and KPV calms inflammation that prevents repair from sticking. And that's why they do really well together for anti inflammatory and injury. I like subcutaneous KPV at 500 micrograms daily for four to eight weeks, then two to four weeks off depending on the the inflammation. Usually that's enough time to like really cover a lot of suppressing the inflammation, but if you need to do it longer, there's nothing wrong with that. It's just one of those things we tend to do better because of the way peptides work. When we cycle them, we're more responsive to them. When we look at an injury stack, I like subcutaneous KPV 500 micrograms daily layered onto standard PPC plus TB 500 injury and you could run this for six to 12 weeks. And it's, I think it's highly underrated for chronic tendinopathies. Installed rehab also too. This is one of those ones. Just use discretion when it comes to cycling because I would rather someone use BPC, TB500 and KPV altogether until the injury is healed rather than cycling off just because they've been on it for eight weeks. I would say hey, use that until you're healed, especially if things are moving in the right direction rather than just cycling off at some arbitrary number. We look at skin and MCAs. I really like compounded cream at 01 to 02 5%. There are research companies out there which I will not name in a public video but, but that now have KPV cream which is really cool. Cuz 2 years ago I was just thinking man, it would be so cool because there's, there's clinical literature around compounded KPV cream but there was no one selling it. I was like man, it would be so cool if you could get KPV into a cream and actually commercialize it. And now thankfully to the work of a lot of really smart and really good people, we do have that. And you could also do this as a micro needle before topical application increases penetration significantly. And then what I would also do is combine it with oral and or subcutaneous for a systemic effect. But it does work really well for skin. Anytime I might have like a pimple breakout here or there, I'll rub some KPV on it and it does really well to bring down that inflammation. For the MCAS protocol I start very low. Maybe even for some of those people. You could start at 250 micrograms once daily oral just to help heal the gut environment and then watch for reactivity. Some patients tolerate doses up to 500 microgram twice daily after titration, especially for those MCAST people. And then you could bring in the injection after. So again if, if there's people that are very sensitive, maybe just start them on the oral and then make sure they do okay after a couple of weeks and then introduce the injectable as well. Timeline, what should we expect? And when I say in week one to two, most people are going to see gut symptoms improve on Oral protocols and people that if they do have any sort of reaction, they would usually settle there, although it's very rare. Weeks 2 to 3 energy injury benefits appear as reduced morning stiffness and faster recovery between sessions. Weeks 3 to 4 Systemic inflammatory effects produce objective change. Usually energy and well being shift, especially if it's someone that has had systemic chronic inflammation via rheumatoid arthritis or Crohn's or something like that. And then week six to eight is typically when we see an inflammatory lab, markers like HSCRP move in the right direction. Skin conditions really usually begin to respond, although they can be highly variable. But kpv, I will say this just to set the expectation and is not a peptide you feel on day one usually is going to be a cumulative effect. Now that doesn't mean if you have an acute flare up, that you won't get benefits on day one. But for someone that is dealing with that chronically over a long period of time, it's not gonna happen overnight. And so you gotta give KPV a chance to work. And usually that's gonna take at least a six to eight week window to do so. Now why should we cycle kpv? It's a very short peptide, it does not have a classic desensitization problem and there's no receptor that can technically desensitize it. However, we cycle for these reasons. One, it's always just out of safety precaution. Just because of peptides, we don't have a long lot of long term data. It also gives us an assessment window. It breaks down to see what KPV is actually doing. And so if symptoms return, you know, the protocol was working and that you could go back on it. And then also we get cumulative exposure. It reduces the cumulative exposure as a conservative precaution until we have longer term data. And I think a lot of times with these peptides it's just something. Do I worry about that with KPV having issues long term? Absolutely not. But we just don't know what we don't know. And I think we talk about peptides, we're always gonna be a little bit more sensitive to it when we cycle on and cycle off. And then we also get the benefit of just having that to where we we don't know what we don't know. And so it translates into making sure that we can monitor ourselves as we go along. Most active protocols cycle for six to eight weeks on and two to four weeks off. This is just kind of standard autoimmune patients. I would Say eight weeks on, four weeks off. These people are oftentimes gonna need those longer cycles. And then you could use long term maintenance. So continuous low dose at 250micrograms daily for chronic conditions with periodic breaks every few months, I think it's totally fine. And then if you're doing the higher dose of acute flares, I like targeted short cycles of two to three weeks for acute flares, then you could step down for maybe a week or two to go back on to the higher dose. Again, this is, it's, it's simple but just use your discretion based on the, the benefits that you need or the benefits you're expecting to get. Let's look at some off cycle expectations. Benefits often persist for several weeks off cycle because again of the cascade that's happening in the body and this is a sign the protocol shifted the tissue meaningly. But again if you cycle off and some of the benefits seem to dwindle, you probably needed to stay on for a longer period of time. If symptoms return quickly, the underlying drivers are still present. Again, consider longer cycles and work on things like diet, sleep and stress. Again, if you go eat, let's say a bunch of fried food and you're expecting KPV to stop the inflammation of the fried food, it's probably going to help that. However, I would recommend that you don't eat fried food three times a day every day because no matter how much KPV you have, you are still overloading the body with inflammation. Kind of the same thing when it comes to injury stuff. I talk to people all the time and I'm like this too because I, I come from an athlete's background, but I talk to people, you know, maybe they have tennis elbow, their knees hurt from playing basketball or something like that. And honestly, if you are using something repetitively is inducing more and more inflammation, no amount of KPV in the world is necessarily going to stop the, the onslaught of inflammation. It can help heal it if you take time off, but again if you're not being intelligent about how you do things, whether it's on the diet side or the lifestyle side, KPV is not going to fix all those things. It can definitely help, but it's not going to fix it. If the benefit holds for weeks after stopping the protocol shift to the tissue meaningly, and that is the assessment value of cycling. It tells you whether KPV was doing real work or whether the underlying condition needed more foundational attention. And the sustained off cycle benefit is the best signal that KPV Is working as intended and hopefully healed the environment going forward. Let's talk about sequencing. So if you have a house on fire, you do not start hanging new drywall while the flames are still active. KPV calms the inflammatory environment. PPC, TB500 and other regenerative peptides do the rebuilding. Both jobs need to get done and the order matters when inflammation is severe. What I like to do first, especially for someone with gut issues, is, is to use KPV 500 micrograms per day to calm inflammation. And then in weeks two, that would be week one and weeks two we could add BPC and continue with the kpv. And then in phase three we would take a break or we would go to a little bit more of an aggressive protocol depending on how the person's doing. But what we want to do with KPV is calm the inflammatory environment before introducing the rebuilder, whether that's BPC, whether it's TB500. And so starting BPC in a severely inflamed gut fights against the backdrop rather than working with it. It's not that it doesn't work, but it just works so much better with KPV for injury. I like kind of the same thing. So 500 micrograms a day sub Q to reduce inflammation for the first week and then we can add in BPC and TB500 to help with that because now we're calming the inflammatory environment. And then by 12 weeks we can say, okay, is this something that I need to continue doing or is this something that I can cycle off because I'm pretty much healed? And a lot of times you can come off of the KPV because you've pretty much stopped the inflammatory environment site. And then same thing, when we start BPC and TB amid high inflammation, it's not always going to work well. And I think that's something now that we have so many more people using peptides that I'm seeing a lot is that just people are using BPC and TB500 thinking they're going to fix everything and a lot of times it's not doing enough to suppress the inflammation. Looking at reconstitution, very simple. Obviously KPV comes to lyophilized freeze dried powder. You have to add back water. Most vials come in 5 to 10 milligrams. If we have a 5 milligram vial, I would say put 2 mls of water. If you have a 10 milligram vial, I Would say put 2 mls of Water. And again, just know the math. If you have a 5 milligram vial that you add 2 milliliters of water, it's now 2.5 milligrams per milliliter. And so if you wanted a 500 microgram dose, that would be 20 units. Very simple. You can see there on the slides if you want to screenshot that. I know some of you guys are like, oh, do you have to talk about this? But it's something that I get asked all the time. Same thing. If I have a 10 milligram vial, add 2 mls of water, now 5 milligrams per milliliter. So if I wanted 500 micrograms, that would be 10 units. And so there you go, there you have it with the dosing again, very simple. With the reconstitution procedure, I've got this on the slides, but you guys know what to do. Just make sure you're shooting the water down the side of the bottle, let it settle, kind of don't shake the bottle, you don't need to shake it or anything like that. It'll mix. And then injection wise, 29 to 31 gauge insulin syringe 5 16, those are the ones that I use. 31 gauge 5 16, they're the easiest. So sub q into the fatty layer just under the skin. I would inject this if you have a site injury next to the site or as close as possible without irritating it, or if you were injecting it for systemic effects, you could just inject into the belly fat and that's usually good enough. And I like to do down at a 45 degree angle wherever I'm injecting for storage and travel. Obviously same thing, very simple with peptides. You know, the, the rule of thumb is used within 30 days. But I've used KPV that is six months old. And so that's what the science is always gonna tell you. But it's just because we don't have a lot of science to validate that they do last for longer. Sure, maybe there is some less potency the longer it sets, but I've used KPV that's six to eight months old and was still fine. And so traveling wise, just obviously keep it cold with cold packs in your insulin cooler. What to track? Let's look at some subjective markers. Basically just how you feel. Obviously look at energy and sleep, energy level and sleep quality rated to 10 each morning. Stiffness and pain, that's going to be the biggest one. Especially if you have arthritis or inflammatory issues in your joint. This could be wonderful for you but morning stiffness is a sensitive early signal. Then gut symptoms obviously that's huge. Bloating, pain, post male energy, stool quality, skin and mood. And also just track your skin quality because that will really help as well. Let's look at some of the objective biomarkers. The biggest one is H H S crp. This is the most sensitive systemic inflammation marker. The and this is what I would primarily watch for people on kpv. A lot of people might not even realize they have a high HSCRP to which I I've seen KPV bring that down from like a five down onto a one which works really well. Ferritin can be a chronic inflammation marker. Elevated ferritin often tracks with inflammatory burden. And so you would wanna look at that if you're using KPV on blood work. CBC with differential which is complete blood count tracks immune cell populations and general health statics. Obviously comprehensive metabolic panel. You won't really see a lot change unless it's an inflammatory thing and then again just condition specific. So there are any specific things like antibodies or things like that. KPV can work really well for those stacking. Let's talk about this because I love stacking it with BPC157. I would say KPV and BPC157 for inflammation is perhaps the highest value pairing in clinical practice outside of again using a glp. BPC rebuilds the tissue through nitric oxide and growth factor pathways. The anti inflammatory effects are secondary. And then we have KPV which provides a primary anti inflammatory layer that BPC lacks. It also directly blocks NF kappa B. And again dosing wise I like these in a one to one ratio. So you could use 250 to 500 micrograms of each one to two times daily for six to eight weeks. And again that would be great for IBD, leaky gut, IBS, post antibiotic damage and chronic tendinopathy and post surgical recovery. Then we could layer in TB500 as well. That's going to add in a remodeling and angiogenesis effect. You could do the the TB, BPC and KPV all in a one to one ratio. Or you could do higher doses of TB 500. Just kind of depends on what you want to do. So use discretion there. If you're getting a blend of them, for instance in the CLO blend which would also have ghk obviously they're usually in a one to one ratio there which is totally fine. Some other peptides that people don't realize that pair really well with kpv. We'll talk about them. One is lorazatide. Lorazatide is a is an oral peptide. It is so underrated too and it was originally developed for celiac disease but the benefits go beyond it even just doing celiac disease. Lorazatide tightens tight junctions while KPV addresses the inflammation driving the leak and their complimentary mechanisms that target the same problem from different angles. KPV and LO37 are amazing together for gut pathogen overgrowth. KPV counterbalances counterbalances LL37's inflammatory tendency which allows the antimicrobial benefits without the inflammatory cost. Not that that really happens if you're doing LL37 at a low dose. But for people that have SIBO or any sort of bacterial issues or viral issues or even mold issues, those two together work amazing because you get the reduction in inflammation from the KPV and then the direct antimicrobial benefit from the LL37. Then we have KPB and thy and alpha 1. For autoimmune conditions we get the TH1 immune balancing plus the inflammation suppression. Again very two complimentary peptides are very synergistic together and I love using those together as well. And then let's talk about trt. There's no interaction. KPV does not affect hormone signaling so you can stack it freely with trt. Although I think it it just works well for people that are on TRT and they still have inflammatory issues cause that happens. And then KPV and GLP1 agonist we have no pharmacological interaction with Ropide, SEMA or retrutide. It may help mitigate the inflammatory component of G GLP1 GI side effects so you can stack freely. I really like for people obviously using GLP to suppress inflammation but for people that do have those GI side effects. We actually just did a call in the private group about how to manage GI side effects on GLPs and I love using KPV to help some of those those issues that people have just because it kind of reduces the flare up that some people have in their gut. So you can use those definitely together and they work really well to suppress inflammation. Let's just talk about some supplement and nutraceutical support. I really love glutamine. That's something I don't talk enough about. I could really do master classes on some of the supplements I use on on a regular basis just to limited by time. But glutamine 5 to 10 grams twice daily. It's the primary fuel for enterocytes, which is the cells that are lining the small intestine. So it really helps with gut intestinal barrier function. We have PEA, which is a mass cell stabilizer. 300 to 600 milligrams per day of PEA works really well. I love that one. We have Zinc and Carine, 75-150mg per day for gastric mucosa support and is well studied for upper GI barrier integrity. And then we have in acetyl glucosamine, 500 to 1,000 milligrams a day for gut barrier substrate. It also provides structural building blocks for the mucosal layer. And so I really love if someone has gut issues pairing these things with kpv. Let's get into some troubleshooting. So what if you say I don't feel anything? One, the dose could be low, too low. And so sometimes maybe that 250 microgram dose is too low and you need to go up to the 500 micrograms to 1000 micrograms range. You can definitely do that. Again beyond a thousand, I just don't see that much more added benefit. Duration could be too short. I mentioned that earlier. You just want to make sure you have a cumulative exposure of at least six to eight weeks to really assess whether it's working or not. Could be the wrong route. Maybe you're just taking the oral version because you're scared of injections. The oral and the sub Q are going to work synergistically together. Obviously you could use either one, but I like using them both together. Obviously just verify source quality and then again, it might be the wrong peptide. If inflammation is not the underlying issue, it's not really, really going to do anything. And again, maybe you have a torn shoulder and use KPV and it reduces inflammation, but it's also still not going to help build the structural area that BPC and TB500 would, which is why they work so well together. Injection site reactions would be probably the most common, but again it's very rare in this case. So just make sure that you're rotating injection sites. There is the possibility that you could get a die off reaction, especially with oral kpv. In dysbiosis, people just reduce the dose by half for a week. Hydrate well and consider binders like charcoal or zeolite. Mild G GI discomfort can come with oral dosing. Again, it's a lot of times it could be cleaning out whatever's in the stomach that could be nasty that could be causing issues there and you could just take the the injection that might not have as bad of an effect and then if you get redness, fever, warmth or pus in indicates possible infection to just stop immediately. Again KPV would be good to benefit infection but if that were to happen, just stop it immediately. When to stop? If you get severe or spreading injection site reactions, persistent GI symptoms beyond two weeks, neurological symptoms, worsening autoimmune condition or any anaphylactic type reaction. Again, these are extremely rare. I would say the worst probably or the most common would be the the worsting autoimmune condition. Just for whatever reason some people are just so sensitive to anything. But I would say relative to other peptides I always put this in there just because it's possible because anything is possible with peptides and I've seen it all or feel like I've seen it all at this point. So just be aware of that. But again very rare in the case of KPV. Let's talk about the legality for a second. In September 2023 it was placed on the category 2 list which blocked compounding pharmacies from making it. Unfortunately, the FDA proposed removal of kpv from category 2 in April of this year along with 11 other peptides in July. It's June as the time I'm filling this. So July 23rd and 24th the PCAC will review KPV, BPC, TB500, MOTC, DIP, Epitalin and CMax to remove from the 503A bulks list. Which means that they will not be FDA approved. But it will now be okay for compounding pharmacies to make them a lot easier for doctors to prescribe them. And again that's kind of our best hope right now with kpv. I doubt it will ever become a pharmaceutical drug. Just some FAQ again BPC versus KPV. BPC is 15amino acids rebuilds tissue through nitric oxide and growth factor pathways. KPV is three amino acids directly blocks enough Kappa baby which is the master inflammatory transcription factor and the anti inflammatory anti inflammatory effects are primary rather than secondary and again they work really well together. When we look at oral versus sub Q the oral is the best. For gut focused work we get the peptide one upregulation and inflamed gut tissue which means the KPV concentrates exactly where we need it and the disease state enhances the delivery of the kpv. Also sub Q is better for systemic effects. So for autoimmune tendon injuries, skin MCAs. It bypasses the Pepti1 competition with other medications in the gut. And again, you can use both of those together if you really want to do it. How long will I feel it? 1 to 2 weeks. Usually you'll see gut symptoms improve. 2 to 4 weeks. Usually systemic effects produce subjective change and then 6 to 8 weeks is typically where we'll end up seeing the labs catch up with the practical effects. And again, just one of those things may take time. And again, with skin conditions, those could be highly variable. In some cases it may take two weeks, in some cases it may take up to several months. KPV is not a peptide you feel on day one. Give a fair trial of at least six to eight weeks before drawing conclusions. If it works for you pregnancy, we just don't know. So I would avoid it. Pediatric use. This is a thing I get asked a lot, especially about kids with inflammation or acne issues, things of that nature. There's no data in children and again, we don't really have anything to say. I personally would feel safe in my own children using it. But again, you got to make the decision for yourself and that's not something I'm going to publicly go recommend to people. And then again, when we look at long term continuous use, we don't have any human data beyond a few months. Periodic breaks recommended every six to 12 months at least at a minimum. As a conservative precaution, I would just not do it every day. Again, not that I'm worried about it, but we just don't know what we don't know. People want to ask a lot of times because of the nature of the structure, will it cause skin darkening because it's an alpha Lana site stimulating hormone peptide? No, it does not activate the melanocortin receptors that drive pigmentation. And again, when we look at full alpha MSH or melan tan 2, those do activate menocortin receptors, but KPV does not activate those. There's no skin darkening, there's no appetite changes, there's no libido shift and there's no flushing. If you do get those side effects, you probably don't really have kpv. If that is because I've used a lot of KPV and I've never had even remotely any of those. What about long Covid and MCAs? It's mechanically plausible but not proven. In trials, practitioner reports describe meaningful improvement and some patients, particularly those with gut symptoms, are persistent. Persistent fatigue tied to inflammation, mcas, anecdotal anecdotally yes. And tons of people that I've seen. The parent alpha MSH has mass cell stabilizing effects that KPV partially retains. I would start very low and watch for reactivity and slow titration matters more than hitting a target dose quickly in this population. And you can always start lower with those people just because they oftentimes are more sensitive. Cost and sourcing usually going to be somewhere in the, you know, 50 ish to 100 depending on the vial size. If you're getting at research from the oral side, usually 100 to 200 bucks per month because the orals tend to be a little bit more expensive. You could obviously use it in a clove blend and save money. That's why those blends are so popular. And yes, they do work together. I'm not someone that tells you you can't inject those specific peptides together. I've done it and they all seem to work for me. And we also have lab data now to back that up that they're all stable even after 30 days of being mixed. And again, just know what you're doing when you are looking at quality indicators. Why isn't KPV more popular? One, it's an economics problem. Obviously it's cheap to synthesize and weak patent protection. So it's not gonna be something that's pharmaceutically introduced or even from a supplement standpoint. There's not gonna be a lot of money made off of it. No pharma company has funded the larger clinical trials that would generate brand recognition and there's no financial incentive to do so. And obviously too there's an awareness problem. The peptide community defaults to BPC and TB500 because those are better known. They have more of a track record that they're better known. They're better just in the common consciousness that people know them. It's not because they are more effective for inflammatory conditions. The result is a peptide that works well, but lives mostly in clinical practice rather than established headlines. We have that Peacock review, hopefully that's a favorable outcome and hopefully that will actually drive many more doctors who are prescribing KPV and many more people to using kpv. I'm hopeful that that go well, but you never know when it comes to government. Let's talk about some next generation compounds as we get close to the end. We have this thing called kdpt, which is a closely related analog with a longer half life. I think this could be really cool. There was a 2011 study on colitis. It's still in research, it's not commercial, it's promising, but this may or may never be brought to market. And again, I think it's cool, but again we don't know. We have this thing called CKPV2 dimer which is stabilized version with antifungal activity which could be really cool. Maybe a little bit of benefits of LL37 without having to take that early clinical interest. But development has been quiet and the structural stability advantage over the monomer. And we have nanoparticle delivery. This is what I was kind of talking about earlier. There was a lab that develops Pepti1 targeted nanoparticles for IBD and the KPV. Plus this thing called FK506 paper is the technical frontier years from approval but it could be really cool with some of these alternative delivery of taking an oral version of KPV but also getting the systemic benefit of it. To sum up, KPV is the peptide that calms the room so the rest of the work can happen. Inflammation is almost always the rate limiting step. In most chronic conditions KPV addresses that inflammation directly at the level of the transcription factor that controls inflammatory gene expression. Again, it's not super flashy, it's not a peptide you feel acutely, but it changes the nature of the body and it helps with infrastructure. And Again, we have 30 years of strong preclinical data across at least eight inflammatory models. We don't have completed phase two or phase three trials in humans. We probably never will get them. But the clinical use rests on the mechanism, preclinical extrapolation and decades of alpha msh, human safety and an uncontrolled practitioner observation and us in the research world obviously that you guys are out there helping move the science forward by using it. There is a ton of anecdotal data that shows this works very very strong. Ryan Reynolds here from Mint Mobile with a message for everyone paying Big Wireless way too much. Please, for the love of everything good in this world, stop with Mint. You can get premium wireless for just $15 a month. Of course if you enjoy overpaying. No judgments. But that's weird. Okay, one judgment anyway. Give it a try@mintmobile.com Switch upfront payment of $45 for three month plan equivalent to $15 per month required Intro rate first three months only, then full price plan options available, taxes and fees extra. See full terms@mintmobile.com and then lastly just use it. Well, just remember with KPV it's a tool if diet, sleep and stressor are managed. KPV is not going to perform to the, to the level that you heard me talk about today. Those fundamentals are in place. However, KPV often does exactly what the mechanism will predict does it releases the inflammatory break and lets the healing happen. And if you ever run a peptide protocol that worked for a while and then plateaued, KPV can oftentimes be the addition most likely to break that plateau. But just remember, it's a tool, it's not a panacea. It's not going to fix everything, but it can help you get going in the right direction if you're doing the, the rest of the work. And that is it for the slides and that is the master class on kpv. Hopefully that was informative to you guys. Again, I loved preparing this, filming this. I just love this whole presentation the, the process that went into it because I'm so passionate about using kpv. It's again one of the peptides that to me I personally rank even over BPC and TB500 in terms of the effectiveness and what it does. Again I, I said it before but if I could choose KPV over those two, I would choose KPV every time because of the amazing anti inflammatory benefits it has. And I will say practically speaking it works faster and better for me to help heal anytime I've experienced inflammation. And again, it's just one of those because of what it does. It's so versatile in the nature where some of those other peptides are extremely, are extremely valuable for what they do or what they treat but they just don't have the versatility of kpv. So I look forward to hearing you guys feedback. Let me know in the comments or messages that you send me what you thought of this one. That seems to be very. I seem to be getting really good feedback on doing these master classes and obviously I love them. I'd love, I love more than nothing. I love more than anything to sit down and do long form videos like this. It's literally what I get excited is what I get out of bed in the morning to do. And hopefully you guys enjoy watching and consuming these as much as I enjoy doing them. Especially in the age of social media and AI where everything has gone to short form and kind of just like try to have the goldfish attention span. I want to do the opposite. So thank you guys so much. Just in closing, I am so, so grateful for the support I get from you guys. Whether you use my coded places, you're on the email list you're in my private group, you share this with your friends and family or even if you never do any of those things, but you just help support through viewing the content. Whatever support you give me goes so far in helping me bring these messages to you. So just know that you are appreciated and your love. I will say that in every single video because it's important to me that you know that. And so I know it gets old for you guys that listen all the time, but thank you guys so much. It goes so far in helping me whatever support you provide to bring these message to you. And I plan on doing it for a very, very, very long time to come. So thank you guys. Look forward to your feedback. Feedback and I will see you in the next one. Peace.